The transport of sperm is dependent upon several factors. The sperm must be capable of propelling themselves through the environment of the female vagina and cervix. This environment, which is under cyclic hormonal control, must be favorable to admit the sperm without destroying them. Finally, the sperm must possess the capability of converting to a form that can penetrate the cell membrane of the egg (capacitation).
Following ejaculation, the semen forms a gel which provides protection for the sperm from the acidic environment of the vagina. The gel is liquefied within 20-30 minutes by enzymes from the prostate gland. This liquefaction is important to free the sperm so transportation may occur. The seminal plasma is left in the vagina. The protected sperm with the greatest motility travel through the layers of cervical mucus that guard the entrance to the uterus. During ovulation, this barrier becomes thinner and changes its acidity creating a friendlier environment for the sperm. The cervical mucus acts as a reservoir for extended sperm survival. Once the sperm have entered the uterus, contractions propel the sperm upward into the fallopian tubes. The first sperm enter the tubes minutes after ejaculation. The first sperm, however, are likely not the fertilizing sperm. Motile sperm can survive in the female reproductive tract for up to 5 days.
Egg transport begins at ovulation and ends once the egg reaches the uterus. Following ovulation, the fimbriated (finger-like) end of the fallopian tube sweeps over the ovary. Adhesive sites on the cilia, which are located on the surface of the fimbriae, are responsible for egg pickup and movement into the tube. The cilia within the tube and muscular contractions resulting from the movement of the egg create a forward motion. Transport through the tube requires about thirty hours. Conditions such as pelvic infections and endometriosis can permanently impair the function of the fallopian tubes, due to scarring and or damage to the fimbriae.
Once the egg arrives at a specific portion of the tube called the ampullar-isthmic junction, it rests for another thirty hours. Fertilization (sperm union with the egg) occurs in this portion of the tube. The fertilized egg then begins a rapid descent to the uterus. The period of rest in the tube appears to be necessary for full development of the fertilized egg and preparation of the uterus for receipt of the egg. Defects in the fallopian tube may impair transport and increase the risk of tubal (ectopic) pregnancy.
Following ovulation, the egg is capable of fertilization for only 12-24 hours. Contact between the egg and sperm is random. A membrane (shell) surrounding the egg, the zona pellucida, has two major functions in fertilization. First, the zona pellucida contains sperm receptors which are specific for human sperm. Second, once the membrane has been penetrated by the sperm, it becomes impermeable to penetration by other sperm.
Following penetration, a series of events set the stage for the first cell division. The single-cell embryo is called a "zygote." Over the course of the next seven days, the human embryo undergoes multiple cell divisions in a process called mitosis. At the end of this transition period, the embryo becomes a mass of very organized cells called a blastocyst. As women get older, it is now believed, this process of early embryo development is increasingly impaired due to diminishing egg quality.
Once the embryo reaches the blastocyst stage (approximately five to six days after fertilization), it hatches out of its zona pellucida (shell) and begins the process of implantation. In nature, 50% of all fertilized eggs are lost before a woman's missed menses. So, too, in the in vitro fertilization (IVF) process an embryo may begin to develop but not make it to the blastocyst stage (the first stage where those cells destined to become the fetus separate from those which will become the placenta). The blastocyst may implant but not grow or the blastocyst may grow and still cease development before the two week time at which a pregnancy can be detected. The receptivity of the uterus and the health of the embryo are important for the implantation process.
|The UCSF Center for Reproductive Health, located in Northern California's San Francisco Bay Area offers a comprehensive array of infertility evaluation and treatment options for both men and women. Our services include: Infertility Evaluation, Male Reproductive Health, Fertility Preservation, Reproductive Surgery, Tubal Reversal Surgery, In Vitro Fertilization (IVF), Intracytoplasmic Sperm Injection (ICSI), Pre-implantation Genetic Diagnosis (PGD), Intrauterine Insemination (IUI), Ovulation Induction, Donor Sperm Insemination, Egg Donor Program for Donors, Egg Donor Program for Recipients, Polycystic Ovarian Syndrome (PCOS), Recurrent Pregnancy Loss, Gestational Surrogacy, Genetic Screening and Counseling Psychological Support.|